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@Article{HollandaLoLaBeCoZa:2014:GrCaNa,
               author = "Hollanda, L. M. and Lobo, A. O. and Lancellotti, M. and Berni, E. 
                         and Corat, Evaldo Jos{\'e} and Zanin, Hudson",
          affiliation = "UNICAMP and UNIVAP and UNICAMP and UNICAMP and {Instituto Nacional 
                         de Pesquisas Espaciais (INPE)} and {Instituto Nacional de 
                         Pesquisas Espaciais (INPE)}",
                title = "Graphene and carbon nanotube nanocomposite for gene transfection",
              journal = "Materials Science and Engineering C",
                 year = "2014",
               volume = "39",
               number = "1",
                pages = "288--298",
                month = "June",
             keywords = "BET adsorption isotherms, carbon nanotube nanocomposites, cell 
                         viability, Fourier transform infra reds, graphene oxides, green 
                         fluorescent protein, high-resolution scanning electron 
                         microscopies, transfection efficiency, carbon nanotubes, cell 
                         culture, Cells, Composite materials, DNA, Fluorescence, Gene 
                         transfer, Genes, Graphene, Nanocomposites, Particle size analysis, 
                         Plasma etching, Plasmas, Scanning electron microscopy, Zeta 
                         potential, Molecular biology.",
             abstract = "Graphene and carbon nanotube nanocomposite (GCN) was synthesised 
                         and applied in gene transfection of pIRES plasmid conjugated with 
                         green fluorescent protein (GFP) in NIH-3T3 and NG97 cell lines. 
                         The tips of the multi-walled carbon nanotubes (MWCNTs) were 
                         exfoliated by oxygen plasma etching, which is also known to attach 
                         oxygen content groups on the MWCNT surfaces, changing their 
                         hydrophobicity. The nanocomposite was characterised by high 
                         resolution scanning electron microscopy; energy-dispersive X-ray, 
                         Fourier transform infrared and Raman spectroscopies, as well as 
                         zeta potential and particle size analyses using dynamic light 
                         scattering. BET adsorption isotherms showed the GCN to have an 
                         effective surface area of 38.5 m2/g. The GCN and pIRES plasmid 
                         conjugated with the GFP gene, forming {\`A}-stacking when 
                         dispersed in water by magnetic stirring, resulting in a helical 
                         wrap. The measured zeta potential confirmed that the plasmid was 
                         connected to the nanocomposite. The NIH-3T3 and NG97 cell lines 
                         could phagocytize this wrap. The gene transfection was 
                         characterised by fluorescent protein produced in the cells and 
                         pictured by fluorescent microscopy. Before application, we studied 
                         GCN cell viability in NIH-3T3 and NG97 line cells using both MTT 
                         and Neutral Red uptake assays. Our results suggest that GCN has 
                         moderate stability behaviour as colloid solution and has great 
                         potential as a gene carrier agent in non-viral based therapy, with 
                         low cytotoxicity and good transfection efficiency.",
                  doi = "10.1016/j.msec.2014.03.002",
                  url = "http://dx.doi.org/10.1016/j.msec.2014.03.002",
                 issn = "0928-4931",
                label = "scopus 2014-05 HollandaLoLaBeCoZa:2014:GrCaNa",
             language = "en",
           targetfile = "1-s2.0-S0928493114001180-main.pdf",
                  url = "http://dx.doi.org/10.1016/j.msec.2014.03.002",
        urlaccessdate = "28 nov. 2020"
}


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