author = "Antonini, Leonardo Marasca and Kothe, Vin{\'{\i}}cius and 
                         Reilly, Gwendolen C. and Owen, Robert and Marcuzzo, Jossano 
                         Saldanha and Malfatti, C{\'e}lia de Fraga",
          affiliation = "{Universidade Federal do Rio Grande do Sul (UFRGS)} and 
                         {Universidade Federal do Rio Grande do Sul (UFRGS)} and 
                         {University of Sheffield} and {University of Sheffield} and 
                         {Instituto Nacional de Pesquisas Espaciais (INPE)} and 
                         {Universidade Federal do Rio Grande do Sul (UFRGS)}",
                title = "Effect of Ti6Al4V surface morphology on the osteogenic 
                         differentiation of human embryonic stem cells",
              journal = "Journal of Materials Research",
                 year = "2017",
               volume = "32",
               number = "20",
                pages = "3811--3821",
                month = "Oct.",
             abstract = "Ti6Al4V alloys usually need to undergo some kind of surface 
                         treatment to enable good bone growth and implant integration. In 
                         this work, three treatments that modify the titanium alloy surface 
                         were studied with the aim of promoting osteogenic differentiation 
                         of human-embryonic-stem-cell-derived mesenchymal progenitors 
                         (hESC-MPs). The surface treatments used were mechanical polishing 
                         and electropolishing for 4 or 12 min in an H2SO4/HF/glycerine 
                         solution. The samples were characterised by atomic force 
                         microscopy, profilometry, X-ray photoelectron spectroscopy, and 
                         wettability. Samples were seeded with hESC-MPs in osteogenic 
                         media, and the cell number and alkaline phosphatase activity were 
                         measured. The electropolishing surface treatments influenced the 
                         nanometric morphology and wettability. However, the 
                         electropolished surfaces contributed in the same way as the 
                         mechanically polished surface to osteogenic differentiation, 
                         indicating that differentiation was strongly influenced by 
                         microroughness, which did not differ among the treatments used in 
                         the present work.",
                  doi = "10.1557/jmr.2017.392",
                  url = "http://dx.doi.org/10.1557/jmr.2017.392",
                 issn = "0884-2914",
             language = "en",
           targetfile = "antonini_effect.pdf",
        urlaccessdate = "25 jan. 2021"