author = "Rocha, Marcelo Carvalho da and Santos, Maur{\'{\i}}cio Beux dos 
                         and Zanella, Renato and Prestes, Osmar Damian and 
                         Gon{\c{c}}alves, Alberto Senra and Schuch, Andr{\'e} Passaglia",
          affiliation = "{Universidade Federal de Santa Maria (UFSM)} and {Universidade 
                         Federal de Santa Maria (UFSM)} and {Universidade Federal de Santa 
                         Maria (UFSM)} and {Universidade Federal de Santa Maria (UFSM)} and 
                         {Instituto Nacional de Pesquisas Espaciais (INPE)} and 
                         {Universidade Federal de Santa Maria (UFSM)}",
                title = "Preserved riparian forest protects endangered forest-specialists 
                         amphibian species against the genotoxic impact of sunlight and 
              journal = "Biological Conservation",
                 year = "2020",
               volume = "249",
                pages = "e108746",
                month = "Sept",
             keywords = "Amphibian decline, DNA damage, Pesticides, UV radiation, 
             abstract = "Although agrochemicals and solar ultraviolet (UV) radiation are 
                         both environmental stressors currently associated with amphibian 
                         decline, little is known about their interactive effects on 
                         amphibian larvae. One potential hazardous consequence of this 
                         interaction could be the increase of chromosomal damage and 
                         induction of cell death, which culminate in tadpole mortality. In 
                         this study, the preservation level of the riparian forest of four 
                         different streams located at the Atlantic Forest in southern 
                         Brazil was evaluated through satellite imagery analysis. The 
                         incidence of solar UVB and UVA radiation were measured with a 
                         radiometer in the amphibian breeding sites, and the presence of 
                         agrochemicals in the water was quantified through liquid 
                         chromatography coupled in tandem to mass spectrometry. Tadpole 
                         population density of two endangered forest-specialist amphibian 
                         species (Boana curupi and Crossodactylus schmidti) was evaluated 
                         in each stream, and the chromosomal damage of the collected larvae 
                         was determined through cytogenetic analysis of blood samples. The 
                         results clearly indicate that the preservation of riparian forest 
                         efficiently blocks the incidence of UV radiation upon the water 
                         surface and reduce by 2.7-fold the entry of agrochemicals from 
                         adjacent agricultural crops. Tadpoles collected in streams with 
                         preserved and continuous riparian forest presented a significantly 
                         lower amount of micronuclei, as well as higher population density 
                         in comparison to tadpoles collected in streams located in 
                         converted agricultural landscape. Therefore, this study 
                         demonstrates that the preservation of the riparian forest has 
                         paramount importance in reducing the impact of environmental 
                         genotoxic agents into lotic breeding sites used by 
                         forestspecialist amphibian species.",
                  doi = "10.1016/j.biocon.2020.108746",
                  url = "http://dx.doi.org/10.1016/j.biocon.2020.108746",
                 issn = "0006-3207",
             language = "en",
           targetfile = "rocha_preserved.pdf",
        urlaccessdate = "14 abr. 2021"