author = "Zimbardi, Ana and Camargo, Priscila de F{\'a}tima and Carli, 
                         Sibeli and Aquino Neto, Sidney and Meleiro, Luana P. and Rosa, 
                         Jos{\'e} C. and Andrade, Adalgisa R. de and Jorge, Jo{\~a}o A. 
                         and Furriel, Rosa P. M.",
          affiliation = "{Universidade de S{\~a}o Paulo (USP)} and {Instituto Nacional de 
                         Pesquisas Espaciais (INPE)} and {Universidade de S{\~a}o Paulo 
                         (USP)} and {Universidade de S{\~a}o Paulo (USP)} and 
                         {Universidade de S{\~a}o Paulo (USP)} and {Universidade de 
                         S{\~a}o Paulo (USP)} and {Universidade de S{\~a}o Paulo (USP)} 
                         and {Universidade de S{\~a}o Paulo (USP)} and {Universidade de 
                         S{\~a}o Paulo (USP)}",
                title = "A high redox potential laccase from pycnoporus sanguineus RP15: 
                         potential application for dye decolorization",
              journal = "International Journal of Molecular Sciences",
                 year = "2016",
               volume = "17",
               number = "5",
                pages = "672",
             keywords = "Pycnoporus sanguineus, solid state fermentation, response surface 
                         methodology, laccase, purification, characterization, 
                         decolorization, remazol brilliant blue R, Reactive blue 4, 
                         bromophenol blue.",
             abstract = "Laccase production by Pycnoporus sanguineus RP15 grown in wheat 
                         bran and corncob under solid-state fermentation was optimized by 
                         response surface methodology using a Central Composite Rotational 
                         Design. A laccase (Lacps1) was purified and characterized and the 
                         potential of the pure Lacps1 and the crude culture extract for 
                         synthetic dye decolorization was evaluated. At optimal conditions 
                         (eight days, 26 \˝C, 18% (w/w) milled corncob, 0.8% (w/w) 
                         NH4Cl and 50 mmol¨ L ´1 CuSO4, initial moisture 4.1 mL¨ g ´1 ), 
                         the laccase activity reached 138.6 \˘ 13.2 U¨ g ´1 . Lacps1 
                         was a monomeric glycoprotein (67 kDa, 24% carbohydrate). Optimum 
                         pH and temperature for the oxidation of 
                         2,2-azino-bis(3-ethylbenzthiazoline-6-sulfonate) (ABTS) were 4.4 
                         and 74.4 \˝C, respectively. Lacps1 was stable at pH 3.08.0, 
                         and after two hours at 5560 \˝C, presenting high redox 
                         potential (0.747 V vs. NHE). ABTS was oxidized with an apparent 
                         affinity constant of 147.0 \˘ 6.4 µmol¨ L ´1 , maximum 
                         velocity of 413.4 \˘ 21.2 U¨ mg´1 and catalytic efficiency 
                         of 3140.1 \˘ 149.6 L¨ mmol´1 ¨ s ´1 . The maximum 
                         decolorization percentages of bromophenol blue (BPB), remazol 
                         brilliant blue R and reactive blue 4 (RB4), at 25 or 40 \˝C 
                         without redox mediators, reached 90%, 80% and 60%, respectively, 
                         using either pure Lacps1 or the crude extract. This is the first 
                         study of the decolorization of BPB and RB4 by a P. sanguineus 
                         laccase. The data suggested good potential for treatment of 
                         industrial dye-containing effluents.",
                  doi = "10.3390/ijms17050672",
                  url = "http://dx.doi.org/10.3390/ijms17050672",
                 issn = "1422-0067",
                label = "lattes: 4809959472520732 2 ZimbardiCCAMRAJF:2016:PoApDy",
             language = "en",
           targetfile = "zimbardi_high.pdf",
        urlaccessdate = "26 jan. 2021"