@Article{HurtadoHCQSDSTBTOCT:2021:CyAnAc,
author = "Hurtado, Carolina Ramos and Hurtado, Gabriela Ramos and Cena,
Gabrielle Lupeti de and Queiroz, Rafaela Campos and Silva,
Alexandre Vieira and Diniz, Milton Faria and Santos, Ver{\^o}nica
Ribeiro dos and Trava-Airoldi, Vladimir Jesus and Baptista,
Maur{\'{\i}}cio da Silva and Tsolekile, Ncediwe and Oluwafemi,
Oluwatobi Samuel and Concei{\c{c}}{\~a}o, K{\'a}tia and Tada,
Dayane Batista",
affiliation = "{Instituto Federal de S{\~a}o Paulo (IFSP)} and {Universidade
Estadual Paulista (UNESP)} and {Universidade Federal de S{\~a}o
Paulo (UNIFESP)} and {Instituto Federal de S{\~a}o Paulo (IFSP)}
and {} and {Helmholtz Zentrum M{\"u}nchen} and {Universidade
Federal de S{\~a}o Paulo (UNIFESP)} and {Instituto Nacional de
Pesquisas Espaciais (INPE)} and {Universidade de S{\~a}o Paulo
(USP)} and {University of Johannesburg} and {University of
Johannesburg} and {Universidade Federal de S{\~a}o Paulo
(UNIFESP)} and {Universidade Federal de S{\~a}o Paulo
(UNIFESP)}",
title = "Diamond nanoparticles-porphyrin mthpp conjugate as
photosensitizing platform: Cytotoxicity and antibacterial
activity",
journal = "Nanomaterials",
year = "2021",
volume = "11",
number = "6",
pages = "e1393",
month = "June",
keywords = "Drug-resistance, Nanoconjugate, Photodynamic antimicrobial therapy
(aPDT), Photosensitizers (PS).",
abstract = "Conjugation of photosensitizers (PS) with nanoparticles has been
largely used as a strategy to stabilize PS in the biological
medium resulting in photosensitizing nanoparticles of enhanced
photoactivity. Herein, (Meso-5, 10, 15, 20-tetrakis
(3-hydroxyphenyl) phorphyryn (mTHPP) was conjugated with diamond
nanoparticles (ND) by covalent bond. Nanoconjugate ND-mTHPP showed
suitable stability in aqueous suspension with 58 nm of
hydrodynamic diameter and Zeta potential of \−23 mV. The
antibacterial activity of ND-mTHPP was evaluated against
Escherichia coli for different incubation times (024 h). The
optimal activity was observed after 2 h of incubation and
irradiation (660 nm; 51 J/cm2 ) performed right after the addition
of ND-mTHPP (100 µg/mL) to the bacterial suspension. The
inhibitory activity was 56% whereas ampicillin at the same
conditions provided only 14% of bacterial growth inhibition. SEM
images showed agglomerate of ND-mTHPP adsorbed on the bacterial
cell wall, suggesting that the antimicrobial activity of ND-mTHPP
was afforded by inducing membrane damage. Cytotoxicity against
murine embryonic fibroblast cells (MEF) was also evaluated and
ND-mTHPP was shown to be noncytotoxic since viability of cells
cultured for 24 h in the presence of the nanoconjugate (100 µg/mL)
was 78%. Considering the enhanced antibacterial activity and the
absence of cytotoxic effect, it is possible to consider the
ND-mTHPP nanoconjugate as promising platform for application in
antimicrobial photodynamic therapy (aPDT).",
doi = "10.3390/nano11061393",
url = "http://dx.doi.org/10.3390/nano11061393",
issn = "2079-4991",
language = "en",
targetfile = "hurtado-diamond.pdf",
urlaccessdate = "03 jun. 2024"
}