@Article{PereiraPupiSaka:2021:EvHyUs,
               author = "Pereira, Douglas Cubas and Pupin, Breno and Sakane, Kumiko 
                         Koibuchi",
          affiliation = "{Universidade do Vale do Para{\'{\i}}ba (UNIVAP)} and {Instituto 
                         Nacional de Pesquisas Espaciais (INPE)} and {Universidade do Vale 
                         do Para{\'{\i}}ba (UNIVAP)}",
                title = "Evaluation of hydrogel use in the development of Rapanea 
                         ferruginea with water restriction by vibrational Fourier Transform 
                         Infrared Spectroscopy (FTIR-UATR)",
              journal = "Ambiente e {\'A}gua",
                 year = "2021",
               volume = "16",
               number = "5",
                month = "Set./Out.",
             keywords = "FTIR infrared spectroscopy, plant water management, superabsorbent 
                         polymer, espectroscopia no infravermelho FTIR, manejo 
                         h{\'{\i}}drico de plantas, pol{\'{\i}}mero, superabsorvente.",
             abstract = "This study evaluated the use of hydrogel on the development of 
                         Rapanea ferruginea under water restriction through Vibrational 
                         Fourier Transform Infrared Spectroscopy (FTIR-UATR). Seedlings of 
                         approximately 30 cm height were transferred in pots with 3 L of 
                         soil. The group of seedlings was separated into 5 different 
                         triplicate treatments according to the amount of hydrogel, as 
                         follows: H1 (25%), H2 (20%), H3 (15%), H4 (10%) and S (control 
                         without hydrogel). The applied hydrogel was mixed homogeneously 
                         with the soil. All treatments were irrigated with 40 mL of water. 
                         FTIR spectra were obtained from fresh leaves collected during 13 
                         months of monitoring. The correlation of the hydroxyl (water) band 
                         with the main biomolecules between treatments with the control was 
                         evaluated using the Mann-Whitney test (p<0.05). The biomolecule 
                         bands were subjected to principal component analysis (PCA) and 
                         hierarchical clustering analysis (HCA). The results indicate a 
                         significant correlation of the water band with the lipid, 
                         hemicellulose, cell wall and starch components in the species. The 
                         use of hydrogel resulted in a significant difference in the water 
                         absorption band in relation to the control group through the 
                         Mann-Whitney test and in biomolecules as the HCA and PCA analysis 
                         suggested. The best development was observed in groups H4 (10%), 
                         H2 (20%) and H3 (15%). The use of hydrogel positively influences 
                         the biomolecular development of the Rapanea ferruginea and 
                         monitoring is viable by FTIR. RESUMO: Objetivou-se avaliar a 
                         influ{\^e}ncia do uso de hidrogel sobre o desenvolvimento da 
                         Rapanea ferruginea sob restri{\c{c}}{\~a}o h{\'{\i}}drica por 
                         meio da t{\'e}cnica de Espectroscopia Vibracional no 
                         Infravermelho M{\'e}dio com Transformada de Fourier (FTIR-UATR). 
                         As mudas com aproximadamente 30 cm de altura foram transferidas em 
                         vasos com 3 L de solo. O grupo de mudas foi separado em 5 
                         tratamentos distintos em triplicata de acordo com a quantidade de 
                         hidrogel, sendo: H1 (25%), H2 (20%), H3 (15%), H4 (10%) e S 
                         (controle sem hidrogel). O hidrogel aplicado foi misturado de 
                         forma homog{\^e}nea ao solo. Todos tratamentos foram irrigados 
                         diariamente com 40 mL de {\'a}gua. Os espectros FTIR foram 
                         obtidos das folhas in natura coletadas por 13 meses de 
                         monitoramento. Foi avaliada a correla{\c{c}}{\~a}o da banda de 
                         hidroxila ({\'a}gua) com as principais biomol{\'e}culas e as 
                         diferen{\c{c}}as existentes entre os tratamentos com o controle 
                         por meio do Teste de Mann-Whitney (p<0,05). As bandas das 
                         biomol{\'e}culas foram submetidas a an{\'a}lise de componentes 
                         principais (PCA) e an{\'a}lise hier{\'a}rquica de agrupamento 
                         (HCA). Os resultados indicam uma correla{\c{c}}{\~a}o 
                         significativa da banda de {\'a}gua com os componentes 
                         lip{\'{\i}}dios, hemicelulose, parede celular e amido na 
                         esp{\'e}cie. O uso do hidrogel proporcionou diferen{\c{c}}a 
                         significativa na banda de absor{\c{c}}{\~a}o de {\'a}gua em 
                         rela{\c{c}}{\~a}o ao grupo controle por meio do Teste de 
                         Mann-Whitney e nas biomol{\'e}culas como a an{\'a}lise de HCA e 
                         PCA sugeriu. O melhor desenvolvimento foi observado nos grupos H4 
                         (10%), H2 (20%) e H3 (15%). O uso do hidrogel influencia 
                         positivamente no desenvolvimento biomolecular da Rapanea 
                         ferruginea e o monitoramento {\'e} vi{\'a}vel por FTIR.",
                  doi = "10.4136/ambi-agua.2744",
                  url = "http://dx.doi.org/10.4136/ambi-agua.2744",
                 issn = "1980-993X",
             language = "en",
           targetfile = "Pereira_evaluation.pdf",
        urlaccessdate = "11 jun. 2024"
}