@Article{LoboSiNeMaCoCo:2013:ViInVi,
               author = "Lobo, Anderson Oliveira and Siqueira, Idalia A. W. B. and Neves, 
                         Marcele F. das and Marciano, Fernanda Roberta and Corat, Evaldo 
                         Jos{\'e} and Corat, Marcus Alexandre F.",
          affiliation = "Laboratory of Biomedical Nanotechnology (NANOBIO), Universidade do 
                         Vale do Paraiba (UNIVAP), Av. Shishima Hifumi 2911, Sao Jose dos 
                         Campos, 12224-000, Brazil and Laboratory of Biomedical 
                         Nanotechnology (NANOBIO), Universidade do Vale do Paraiba 
                         (UNIVAP), Av. Shishima Hifumi 2911, Sao Jose dos Campos, 
                         12224-000, Brazil and Laboratory of Biomedical Nanotechnology 
                         (NANOBIO), Universidade do Vale do Paraiba (UNIVAP), Av. Shishima 
                         Hifumi 2911, Sao Jose dos Campos, 12224-000, Brazil and Laboratory 
                         of Biomedical Nanotechnology (NANOBIO), Universidade do Vale do 
                         Paraiba (UNIVAP), Av. Shishima Hifumi 2911, Sao Jose dos Campos, 
                         12224-000, Brazil and {Instituto Nacional de Pesquisas Espaciais 
                         (INPE)} and Centro Multidisciplinar para Investiga{\c{c}}{\~a}o 
                         Biologica (CEMIB), Universidade Estadual de Campinas (UNICAMP), 
                         Rua 5 de Junho, s/n, Cidade Universitaria, Campinas, 13083-877, 
                         Brazil",
                title = "In vitro and in vivo studies of a novel 
                         nanohydroxyapatite/superhydrophilic vertically aligned carbon 
                         nanotube nanocomposites",
              journal = "Journal of Materials Science: Materials in Medicine",
                 year = "2013",
               volume = "24",
               number = "7",
                pages = "1723--1732",
                month = "July",
             keywords = "carbon nanotubes, in vitro studies, hydroxyapatite, hydrophilic 
                         compounds, extracellular matrix, bone regeneration.",
             abstract = "An association between in vitro and in vivo studies has been 
                         demonstrated for the first time, using a novel 
                         nanohydroxyapatite/superhydrophilic vertically aligned multiwalled 
                         carbon nanotube (nHAp/VAMWCNT-O2) nanocomposites. Human osteoblast 
                         cell culture and bone defects were used to evaluate the in vitro 
                         extracellular matrix (ECM) calcification process and bone 
                         regeneration, respectively. The in vitro ECM calcification process 
                         of nHAp/VAMWCNT-O2 nanocomposites were investigated using alkaline 
                         phosphatase assay. The in vivo biomineralization studies were 
                         carried out on bone defects of C57BL/6/JUnib mice. Scanning 
                         electron microscopy, micro-energy dispersive spectroscopy, X-ray 
                         photoelectron spectroscopy, and X-ray difractometry analyses 
                         confirmed the presence of the nHAp crystals. nHAp/VAMWCNT-O2 
                         nanocomposites induced in vitro calcification of the ECM of human 
                         osteoblast cells in culture after only 24 h. Bone regeneration 
                         with lamellar bone formation after 9 weeks was found in the in 
                         vivo studies. Our findings make these new nanocomposites very 
                         attractive for application in bone tissue regeneration.",
                  doi = "10.1007/s10856-013-4929-y",
                  url = "http://dx.doi.org/10.1007/s10856-013-4929-y",
                 issn = "0957-4530",
                label = "scopus",
             language = "en",
           targetfile = "10.1007_s10856-013-4929-y.pdf",
        urlaccessdate = "02 maio 2024"
}