@Article{HurtadoHCQSDSTBTOCT:2021:CyAnAc,
               author = "Hurtado, Carolina Ramos and Hurtado, Gabriela Ramos and Cena, 
                         Gabrielle Lupeti de and Queiroz, Rafaela Campos and Silva, 
                         Alexandre Vieira and Diniz, Milton Faria and Santos, Ver{\^o}nica 
                         Ribeiro dos and Trava-Airoldi, Vladimir Jesus and Baptista, 
                         Maur{\'{\i}}cio da Silva and Tsolekile, Ncediwe and Oluwafemi, 
                         Oluwatobi Samuel and Concei{\c{c}}{\~a}o, K{\'a}tia and Tada, 
                         Dayane Batista",
          affiliation = "{Instituto Federal de S{\~a}o Paulo (IFSP)} and {Universidade 
                         Estadual Paulista (UNESP)} and {Universidade Federal de S{\~a}o 
                         Paulo (UNIFESP)} and {Instituto Federal de S{\~a}o Paulo (IFSP)} 
                         and {} and {Helmholtz Zentrum M{\"u}nchen} and {Universidade 
                         Federal de S{\~a}o Paulo (UNIFESP)} and {Instituto Nacional de 
                         Pesquisas Espaciais (INPE)} and {Universidade de S{\~a}o Paulo 
                         (USP)} and {University of Johannesburg} and {University of 
                         Johannesburg} and {Universidade Federal de S{\~a}o Paulo 
                         (UNIFESP)} and {Universidade Federal de S{\~a}o Paulo 
                         (UNIFESP)}",
                title = "Diamond nanoparticles-porphyrin mthpp conjugate as 
                         photosensitizing platform: Cytotoxicity and antibacterial 
                         activity",
              journal = "Nanomaterials",
                 year = "2021",
               volume = "11",
               number = "6",
                pages = "e1393",
                month = "June",
             keywords = "Drug-resistance, Nanoconjugate, Photodynamic antimicrobial therapy 
                         (aPDT), Photosensitizers (PS).",
             abstract = "Conjugation of photosensitizers (PS) with nanoparticles has been 
                         largely used as a strategy to stabilize PS in the biological 
                         medium resulting in photosensitizing nanoparticles of enhanced 
                         photoactivity. Herein, (Meso-5, 10, 15, 20-tetrakis 
                         (3-hydroxyphenyl) phorphyryn (mTHPP) was conjugated with diamond 
                         nanoparticles (ND) by covalent bond. Nanoconjugate ND-mTHPP showed 
                         suitable stability in aqueous suspension with 58 nm of 
                         hydrodynamic diameter and Zeta potential of \−23 mV. The 
                         antibacterial activity of ND-mTHPP was evaluated against 
                         Escherichia coli for different incubation times (024 h). The 
                         optimal activity was observed after 2 h of incubation and 
                         irradiation (660 nm; 51 J/cm2 ) performed right after the addition 
                         of ND-mTHPP (100 µg/mL) to the bacterial suspension. The 
                         inhibitory activity was 56% whereas ampicillin at the same 
                         conditions provided only 14% of bacterial growth inhibition. SEM 
                         images showed agglomerate of ND-mTHPP adsorbed on the bacterial 
                         cell wall, suggesting that the antimicrobial activity of ND-mTHPP 
                         was afforded by inducing membrane damage. Cytotoxicity against 
                         murine embryonic fibroblast cells (MEF) was also evaluated and 
                         ND-mTHPP was shown to be noncytotoxic since viability of cells 
                         cultured for 24 h in the presence of the nanoconjugate (100 µg/mL) 
                         was 78%. Considering the enhanced antibacterial activity and the 
                         absence of cytotoxic effect, it is possible to consider the 
                         ND-mTHPP nanoconjugate as promising platform for application in 
                         antimicrobial photodynamic therapy (aPDT).",
                  doi = "10.3390/nano11061393",
                  url = "http://dx.doi.org/10.3390/nano11061393",
                 issn = "2079-4991",
             language = "en",
           targetfile = "hurtado-diamond.pdf",
        urlaccessdate = "20 maio 2024"
}