@Article{PereiraPupiBorm:2024:InSaPr,
               author = "Pereira, Douglas Cubas and Pupin, Breno and Borma, Laura de 
                         Simone",
          affiliation = "{Instituto Nacional de Pesquisas Espaciais (INPE)} and {Instituto 
                         Nacional de Pesquisas Espaciais (INPE)} and {Instituto Nacional de 
                         Pesquisas Espaciais (INPE)}",
                title = "Influence of sample preparation methods on FTIR spectra for 
                         taxonomic identification of tropical trees in the Atlantic 
                         forest",
              journal = "Heliyon",
                 year = "2024",
               volume = "10",
               number = "5",
                pages = "e27232",
                month = "Mar.",
             keywords = "Atlantic forest, FTIR, Multivariate analysis, Plant taxonomy, 
                         Tropical trees.",
             abstract = "The Atlantic forest is one of the world's major tropical biomes 
                         due to its rich biodiversity. Its vast diversity of plant species 
                         poses challenges in floristic surveys. Fourier transform infrared 
                         spectroscopy (FTIR) enables rapid and residue-free data 
                         collection, providing diverse applications in organic sample 
                         analysis. FTIR spectra quality depends on the sample preparation 
                         methodology. However, no research on FTIR spectroscopy methodology 
                         for taxonomy has been conducted with tropical tree species. Hence, 
                         this study addresses the sample preparation influence on FTIR 
                         spectra for the taxonomic classification of 12 tree species 
                         collected in the Serra do Mar State Park (PESM) - Cunha Nucleus 
                         S{\~a}o Paulo State, Brazil. Spectra were obtained from intact 
                         fresh (FL), intact dried (DL), and heat-dried ground (GL) leaves. 
                         The spectra were evaluated through chemometrics using Principal 
                         Component Analysis (PCA), Hierarchical Cluster Analysis (HCA), and 
                         Linear Discriminant Analysis (LDA) with validation by LDA-PCA. The 
                         results demonstrate that sample preparation directly influences 
                         tropical species FTIR spectra categorization capability. The best 
                         taxonomic classification result for all techniques, validated by 
                         LDA-PCA, was obtained from GL. FTIR spectra evaluation through 
                         PCA, HCA, and LDA allow for the observation of phylogenetic 
                         relationships among the species. FTIR spectroscopy proves to be a 
                         viable technique for taxonomic evaluation of tree species in 
                         floristic exploration of tropical biomes which can complement 
                         traditional tools used for taxonomic studies.",
                  doi = "10.1016/j.heliyon.2024.e27232",
                  url = "http://dx.doi.org/10.1016/j.heliyon.2024.e27232",
                 issn = "2405-8440",
             language = "en",
           targetfile = "1-s2.0-S2405844024032638-main.pdf",
        urlaccessdate = "05 maio 2024"
}