| 1. Identity statement | ||||
| Reference Type | Journal Article | |||
| Site | mtc-m16d.sid.inpe.br | |||
| Holder Code | isadg {BR SPINPE} ibi 8JMKD3MGPCW/3DT298S | |||
| Identifier | 8JMKD3MGP7W/3986SES | |||
| Repository | sid.inpe.br/mtc-m19/2011/02.22.14.01 (restricted access) | |||
| Last Update | 2011:02.22.14.08.57 (UTC) administrator | |||
| Metadata Repository | sid.inpe.br/mtc-m19/2011/02.22.14.01.06 | |||
| Metadata Last Update | 2018:06.05.04.24.22 (UTC) administrator | |||
| Secondary Key | INPE--PRE/ | |||
| DOI | 10.1016/j.dsr2.2010.10.013 | |||
| ISSN | 0967-0645 | |||
| Citation Key | NakayamaKuArAlFeVaPe:2011:ReArDi | |||
| Title | Revealing archaeal diversity patterns and methane fluxes in Admiralty Bay, King George Island, and their association to Brazilian Antarctic Station activities  | |||
| Year | 2011 | |||
| Month | Jan. | |||
| Access Date | 2024, Apr. 28 | |||
| Secondary Type | PRE PI | |||
| Number of Files | 1 | |||
| Size | 1252 KiB | |||
| 2. Context | ||||
| Author | 1 Nakayama, C. R 2 Kuhn, E 3 Araujo, ACV 4 Alvala, PC 5 Ferreira, WJ 6 Vazoller, R. F 7 Pellizari, V. H | |||
| Group | 1 2 3 4 DGE-CEA-INPE-MCT-BR 5 DGE-CEA-INPE-MCT-BR | |||
| Affiliation | 1 Laboratório de Ecologia de Micro-Organismos, Instituto Oceanográfico, Universidade de São Paulo-IOUSP, Praça do Oceanográfico, 191, 05508-120, São Paulo, SP, Brazil 2 Laboratório de Ecologia de Micro-Organismos, Instituto Oceanográfico, Universidade de São Paulo-IOUSP, Praça do Oceanográfico, 191, 05508-120, São Paulo, SP, Brazil 3 Laboratório de Ecologia de Micro-Organismos, Instituto Oceanográfico, Universidade de São Paulo-IOUSP, Praça do Oceanográfico, 191, 05508-120, São Paulo, SP, Brazil 4 Instituto Nacional de Pesquisas Espaciais (INPE) 5 Instituto Nacional de Pesquisas Espaciais (INPE) 6 Laboratório de Ecologia de Micro-Organismos, Instituto Oceanográfico, Universidade de São Paulo-IOUSP, Praça do Oceanográfico, 191, 05508-120, São Paulo, SP, Brazil 7 Laboratório de Ecologia de Micro-Organismos, Instituto Oceanográfico, Universidade de São Paulo-IOUSP, Praça do Oceanográfico, 191, 05508-120, São Paulo, SP, Brazil | |||
| Journal | Deep-Sea Research. Part 2. Topical Studies in Oceanography | |||
| Volume | 58 | |||
| Number | 1-2 Special Issue: SI | |||
| Pages | 128–138 | |||
| History (UTC) | 2011-07-25 14:12:17 :: marciana :: 2010 -> 2011 2011-08-04 13:42:29 :: marciana -> administrator :: 2011 2011-10-10 00:44:02 :: administrator -> marciana :: 2011 2011-11-04 12:47:41 :: marciana -> administrator :: 2011 2011-11-07 02:55:47 :: administrator -> marciana :: 2011 2011-11-10 13:00:38 :: marciana -> administrator :: 2011 2018-06-05 04:24:22 :: administrator -> tereza@sid.inpe.br :: 2011 | |||
| 3. Content and structure | ||||
| Is the master or a copy? | is the master | |||
| Content Stage | completed | |||
| Transferable | 1 | |||
| Content Type | External Contribution | |||
| Version Type | publisher | |||
| Keywords | Archaea Methane Methanogenesis Molecular diversity 16SrDNA DGGE Sediment Wastewater Admiralty Bay Antarctica Brazilian Antarctic Station POLYMERASE-CHAIN-REACTION AMAZON RIVER FLOODPLAIN SOUTH SHETLAND ISLANDS 16S RIBOSOMAL-RNA METHANOGENIC ARCHAEA MARINE-SEDIMENTS NITROUS-OXIDE PHYLOGENETIC CHARACTERIZATION BIOGEOCHEMICAL PROCESSES SUBSEAFLOOR SEDIMENTS | |||
| Abstract | The study of Antarctic archaeal communities adds information on the biogeography of this group and helps understanding the dynamics of biogenic methane production in such extreme habitats. Molecular methods were combined to methane flux determinations in Martel Inlet, Admiralty Bay, to assess archaeal diversity, to obtain information about contribution of the area to atmospheric methane budget and to detect possible interferences of the Antarctic Brazilian Station Comandante Ferraz (EACF) wastewater discharge on local archaeal communities and methane emissions. Methane fluxes in Martel Inlet ranged from 3.2 to 117.9 ìmol CH4 m-2 d-1, with an average of 51.3±8.5 ìmol CH4 m-2 d-1 and a median of 57.6 ìmol CH4 m-2d-1. However, three negative fluxes averaging -11.3 ìmol CH4 m-2 d-1 were detected in MacKellar Inlet, indicating that Admiralty Bay can be either a source or sink of atmospheric methane. Denaturing gradient gel electrophoresis (DGGE) showed that archaeal communities at EACF varied with depth and formed a group separated from the reference sites. Granulometric analysis indicated that differences observed may be mostly related to sediment type. However, an influence of wastewater input could not be discarded, since higher methane fluxes were found at CF site, suggesting stimulation of local methanogenesis. DGGE profile of the wastewater sample grouped separated from all other samples, suggesting that methanogenesis stimulation may be due to changes in environmental conditions rather than to the input of allochtonous species from the wastewater. 16S ribosomal DNA clone libraries analysis showed that all wastewater sequences were related to known methanogenic groups belonging to the hydrogenotrophic genera Methanobacterium and Methanobrevibacter and the aceticlastic genus Methanosaeta. EACF and Botany Point sediment clone libraries retrieved only groups of uncultivated Archaea, with predominance of Crenarchaeota representatives (MCG, MG1, MBG-B, MBG-C and MHVG groups). Euryarchaeota sequences found were mostly related to the LDS and RC-V groups, but MBG-D and DHVE-5 were also present. No representatives of cultivated methanogenic groups were found, but coverage estimates suggest that a higher number of clones would have to be analyzed in order to cover the greater archaeal diversity of Martel Inlet sediment. Nevertheless, the analysis of the libraries revealed groups not commonly found by other authors in Antarctic habitats and also indicated the presence of groups of uncultivated archaea previously associated to methane rich environments or to the methane cycle. © 2010 Elsevier Ltd. All rights reserved. | |||
| Area | CEA | |||
| Arrangement | urlib.net > BDMCI > Fonds > Produção anterior à 2021 > DIDGE > Revealing archaeal diversity... | |||
| doc Directory Content | access | |||
| source Directory Content | there are no files | |||
| agreement Directory Content |
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| 4. Conditions of access and use | ||||
| Language | en | |||
| Target File | nakayama.pdf | |||
| User Group | administrator marciana | |||
| Visibility | shown | |||
| Archiving Policy | denypublisher denyfinaldraft24 | |||
| Read Permission | deny from all and allow from 150.163 | |||
| Update Permission | not transferred | |||
| 5. Allied materials | ||||
| Mirror Repository | sid.inpe.br/mtc-m19@80/2009/08.21.17.02.53 | |||
| Next Higher Units | 8JMKD3MGPCW/3EU29DP | |||
| Dissemination | WEBSCI; PORTALCAPES; MGA; COMPENDEX. | |||
| Host Collection | sid.inpe.br/mtc-m19@80/2009/08.21.17.02 | |||
| 6. Notes | ||||
| Empty Fields | alternatejournal archivist callnumber copyholder copyright creatorhistory descriptionlevel e-mailaddress electronicmailaddress format isbn label lineage mark nextedition notes orcid parameterlist parentrepositories previousedition previouslowerunit progress project readergroup resumeid rightsholder schedulinginformation secondarydate secondarymark session shorttitle sponsor subject tertiarymark tertiarytype typeofwork url | |||
| 7. Description control | ||||
| e-Mail (login) | tereza@sid.inpe.br | |||
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